Tuition comes to 27,000 USD per year.
This short course will give you a hands-on introduction to the production of biopharmaceutical proteins in mammalian cells. Through a mix of hands-on lab, theoretical exercises and lectures, the course will guide you through topics on mammalian cell cultivation, bioprocess modes, bioprocess control, cell line engineering, single-use equipment, and with a particular focus on perfusion bioprocess. Upon completion of the course, you will have sufficient overview, theoretical knowledge, and practical hands-on experience to e.g., get more company-specific training; better understand and support the upstream department; or to participate in management level decision-making on the covered topics. In addition to extensive hands-on experience with the process, this course will also address many of the common concerns when implementing single-use processes – i.e., sterile connections, environmental concerns/carbon footprint, business economics, and validation. This course was previously called: Introduction to Mammalian Bioprocessing Course schedule Day 1 Lecture 1: Bioreactors: Purpose, history and types Lab 1 Team 1: Thaw, TubeSpin, Glass 1L Team 2: 2L SU batch, fed batch and perfusion Lecture 2: Single use vs stainless steel Lab 2 Team 2: Thaw, TubeSpin, Glass 1L Team 1: 2L SU batch, fed batch and perfusion Day 2 Lecture 3: Bioprocess: Modes and concepts Lecture 4: Controls: CQA, CPP, PID, PAT Lab 3 Team 1: 2L SU batch, fed batch and perfusion Team 2: Thaw, TubeSpin, Glass 1L Lecture 5: Harvesting Lab 4: Team 2: 2L SU batch, fed batch and perfusion Team 1: Thaw, TubeSpin, Glass 1L Day 3 Lab 5 Team 1: Wave and 50L Team 2: Sampling and AMBR Lecture 6: Perfusion Lab 6 Team 2: Sampling and AMBR Team 1: Wave and 50L Lecture 7: Industry Example * The course schedule is subject to possible adjustments. Learning outcomes At the end of this course, participants should be able to: Design of animal cell culture bioreactors, including suspension and immobilized cell bioreactors, conventional stainless steel stirred tanks, and disposable (single-use) bioreactors Identify advantages and disadvantages of single-use technologies relative to multi-use equipment Understand the pros and cons for bioreactor types (rocker, stirred tank, microcarrier) and strategies (batch, fed-batch, perfusion) Setup and operation of single-use options for upstream manufacturing (rocker, stirred tank and perfusion) Advanced automation and control of animal cell culture processes, including bioreactor Process Analytical Technology (PAT) Basic cost calculations to compare single-use options to more traditional multi-use set ups, to provide justification for single-use technologies. Who should attend? This course is intended for participants who are: Professionals within the biopharmaceutical industry who want to gain hands-on experience with mammalian bioprocessing equipment Professionals who want to gain an understanding of upstream mammalian bioprocessing Professionals working indirectly with mammalian bioprocessing who want to better understand it Prerequisites Participants do not need to have specific knowledge in the field of biotechnology other than a basic high-school level understanding of natural sciences. Important information Here you will find important information for short course participants regarding cancellation policies, location and waitlist policies. Registration Course dates will be announced in December 2025 In case, you have any questions related to your registration, please contact Khaled Chatila ( khachat@dtu.dk ). Discount for start-ups and academics We offer employees in start-up companies, educational institutions and the public sector a 25% course fee reduction. Tick off the relevant boxes in the registration form to apply for this discount. You will get to the registration form when adding the course to the basket. Companies wishing to arrange a tailored/private course are more than welcome to contact us to discuss this opportunity. Course instructor Lasse Ebdrup Pedersen Senior Researcher Dr. Pedersen has over a decade of experience working with mammalian cell cultures and has previously led efforts in CHO cell line engineering and manufacturing at the Novo Nordisk Foundation center for Biosustainability. Currently, as senior scientist at DTU Bioengineering, he is co-leading efforts in a cross-Atlantic partnership focused on improving biomanufacturing methods and providing training in the field. Read more
